Resistance to Methotrexate Due to AcrAB-Dependent Export from Escherichia coli
نویسندگان
چکیده
منابع مشابه
Resistance to methotrexate due to AcrAB-dependent export from Escherichia coli.
Many laboratory strains of Escherichia coli are resistant to methotrexate (MTX), a folate analogue that binds dihydrofolate reductase (DHFR). Mutations that inactivate either tolC or acrA confer MTX sensitivity. Further, overexpression of a fusion protein with DHFR activity reverses this sensitivity by titrating out intracellular MTX. These results suggest that MTX accumulates in cells where mu...
متن کاملContribution of the AcrAB-TolC efflux pump to high-level fluoroquinolone resistance in Escherichia coli isolated from dogs and humans.
Fluoroquinolone resistance is mainly caused by mutations in quinolone resistance-determining regions of DNA gyrase and topoisomerase IV in Escherichia coli. The AcrAB-TolC efflux pump contributes to resistance against fluoroquinolone and other antimicrobials. In this study, we investigated a high-level mechanism of fluoroquinolone resistance in E. coli that was isolated from human clinical samp...
متن کاملMethotrexate binding to dihydrofolate reductase from a methotrexate-resistant strain of Escherichia coli.
Activity and fluorescence studies have been performed on dihydrofolate reductase from a methotrexate-resistant strain of Escherichia coli B. Whereas the activity of this enzyme does not depend on the sequence of addition of substrate and cofactor, inhibition by methotrexate has been found to be dependent on preincubation conditions, as well as on ionic strength and dihydrofolate concentration. ...
متن کاملEmphysematous Osteomyelitis due to Escherichia coli
Emphysematous osteomyelitis, especially that involving the extra-axial skeleton, is an extremely rare presentation but associated with significant morbidity and mortality. Here, we report a case in which a 58-year-old female patient with diabetes mellitus presented with emphysematous osteomyelitis that involved the sternum, clavicle, and pelvic bone and was caused by Escherichia coli via hemato...
متن کاملResistance to Aminoglycoside Antibiotics in Escherichia coli Isolated from Urinary Tract Infection in Iran in 2000-2012: A Systematic Review
Background and Objectives: Resistance to aminoglycosides has become a major problem in the treatment of urinary tract infection due to the extensive use of this antibiotic group. Due to the increased resistance of Escherichia coli to aminoglycosides, this study systematically evaluated the resistance to aminoglycosides by Escherichia coli isolated from urinary tract infection (UTI) in Ira...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Antimicrobial Agents and Chemotherapy
سال: 2000
ISSN: 0066-4804,1098-6596
DOI: 10.1128/aac.44.11.3210-3212.2000